Glomerular routing of tumor-derived extracellular vesicles substantiates urinary biopsy - Science | AAAS

We’re sharing this article early to provide faster access to peer-reviewed, accepted research. It is citable and carries a permanent DOI. This version is subject to further edits and will be replaced automatically by the final Version of Record. All legal disclaimers apply. AbstractDiet-induced obesity is associated with impaired satiety signaling and alterations in intestinal microbial communities. Plant-derived extracellular vesicles have recently emerged as bioactive dietary vesicles capable of influencing host physiology. However, the in vivo metabolic effects of Panax ginseng–derived extracellular vesicles (GDE) remain incompletely characterized. Male C57BL/6 mice were fed a low-fat diet, high-fat diet (HF), or HF supplemented with orally administered GDE (3 × 10⁹ particles, 3 times/week) for 9 weeks. Relative epididymal fat pad weight, adipose tissue histology, cholecystokinin (CCK)-mediated satiety responsiveness, gut microbiota composition, and selected intestinal inflammatory markers were evaluated. GDE administration reduced adipocyte diameter and normalized epididymal leptin mRNA expression in HF-fed mice despite comparable energy intake between HF and HF/GDE groups.

Denham, J. & Spencer, S. J. Emerging roles of extracellular vesicles in the intercellular communication for exercise-induced adaptations. Am. J. Physiol. Endocrinol. Metab. 319, E320–E329 (2020). Article Zaborowski, M. P., Balaj, L., Breakefield, X. O. & Lai, C. P. Extracellular vesicles: composition, biological relevance, and methods of study. Bioscience 65, 783–797 (2015). Article

Yeast cells undergoing sexual reproduction, observed by fluorescence microscopy; the green signal reveals a cell wall damage sensor at the site of fusion. Credit: J. Coronas-Serna, UNIGE The fusion of two cells is a decisive event in the sexual reproduction of many organisms. In fungi, cells must not only bring their membranes together and merge them, but also locally open their protective cell wall.