Comparative assessment of phytoplankton community using different filtration methods based on environmental DNA metabarcoding—a case study of Caohai Wetland - Nature
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AbstractIn environmental DNA (eDNA) metabarcoding, the choice of filter membrane material and pore size governs DNA enrichment, detection sensitivity, and taxonomic representativeness of aquatic communities. This study employed eDNA metabarcoding to evaluate the influence of various filter membranes on the phytoplankton community within Caohai Wetland. Four membrane materials with a pore size of 0.22 μm were tested: mixed cellulose esters (MCE), polyethersulfone (PES), nylon (NY), and polytetrafluoroethylene (PTFE), and three pore sizes were tested with the MCE membranes: 0.22 μm, 0.45 μm, and 0.8 μm. The results demonstrated that among the tested materials, MCE filters achieved the best detection performance, yielding higher eDNA recovery, taxonomic counts, class coverage, and Chao1 richness than PES, NY, and PTFE membranes, owing to their superior retention of eDNA and algal particles.
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