A genetically encoded device for transcriptome storage in mammalian cells - Science | AAAS

We’re sharing this article early to provide faster access to peer-reviewed, accepted research. It is citable and carries a permanent DOI. This version is subject to further edits and will be replaced automatically by the final Version of Record. All legal disclaimers apply. AbstractErigeron breviscapus is a widely utilized medicinal herb in traditional Chinese medicine, characterized by high annual demand and notable pharmacological activities. Light intensity is a critical environmental factor influencing secondary metabolism in medicinal plants. However, the effects of light intensity on the accumulation of active ingredients in E. breviscapus remain largely unexplored. In this study, we examined the transcriptomic and metabolomic responses of E. breviscapus to five levels of light intensity (50, 100, 200, 400, 800 μmol·m−2·s−1). The results revealed that higher light intensity (EB-L800) significantly increased the accumulation of scutellarin, chlorogenic acid, isochlorogenic acid A, and isochlorogenic acid C. A total of 1,334 metabolites were detected, and 127.10 Gb of high-quality clean data were generated through transcriptomic sequencing.