Single-cell multiomics and chromatin structure reveal gene-regulatory dynamics in heart failure - Science | AAAS

AcknowledgementsWe thank Rie Shimano, Yukari Azuma, Eriko Tanaka, Mizuki Shinoda, Akie Takahashi, Hiroko Egashira, and Taeko Kawakami for technical support for molecular genetics and Ayami Furuta for technical support for RNA-seq. Seeds for SALK lines and auxin-related reporters were obtained from ABRC. FundingThis work was supported by a JSPS KAKENHI Grant-in-Aid for JSPS Fellows (24KJ1694) to Y. F.; a JSPS KAKENHI Grant-in-Aid for Transformative Research Areas (A) (25H01834) to T. K.; the Cross-ministerial Strategic Innovation Promotion Program (SIP), “Technologies for Smart Bio-industry and Agriculture,” funded by the Cabinet Office, Government of Japan, to Y. I.; a JSPS KAKENHI Grant-in-Aid for Scientific Research (C) (23K05809) to T. S.; the U. S. National Science Foundation (IOS-2319036) to D. W.; a JSPS KAKENHI Grant-in-Aid for Transformative Research Areas (A) (22H05176) and a Grant-in-Aid for Scientific Research (A) (26H02380) to T.

We’re sharing this article early to provide faster access to peer-reviewed, accepted research. It is citable and carries a permanent DOI. This version is subject to further edits and will be replaced automatically by the final Version of Record. All legal disclaimers apply. AbstractFOXO transcription factors are critical for cellular homeostasis and the stress response and have been implicated in longevity in several species. Yet, how these factors function at the chromatin level in differing conditions in human cells is poorly understood. Here, we take an integrated multi-omics approach to identify the chromatin-level mechanisms by which FOXO3 coordinates transcriptional programs in replicating cells and in a model of cellular senescence. In a DNA-damage induced senescence, FOXO3 levels are elevated early in senescence entry. Increased FOXO3 expression induces expression of chromatin remodeling factors, and impacts chromatin accessibility across the genome together with SWI/SNF chromatin remodelers.

Mud from a fjord shows an Atlantic air pattern brought wetter, colder spells to southern Greenland, a clue to how its ice may fare. Greenland’s ice is shrinking as the planet warms. But in the island’s southwest, rainfall and snowfall have been influenced by the same North Atlantic air-pressure pattern for at least 5,000 years. When that pattern tipped one way, the region turned wetter and colder. The record comes from mud on the floor of a Greenland fjord, and it suggests snowfall, not only temperature, shaped how the area’s ice grew and shrank. It doesn’t yet show how glaciers there will respond to the warming ahead. Johan Faust, a researcher at MARUM, the Center for Marine Environmental Sciences at the University of Bremen in Germany, led the work with colleagues in Germany, Norway, Denmark and Canada. A seesaw in the airThe pattern is called the North Atlantic Oscillation. It’s the difference in air pressure between a low that sits near Iceland and a high near the Azores, and it’s strongest in winter. When that difference is large, the pattern is in its positive phase. Northwestern Europe and southwestern Greenland both get more rain and snow.

Lawrence Livermore National Laboratory researcher Isaiah Santistevan led the development of three-dimensional simulations exploring how a nuclear device could deflect or disrupt an asteroid threatening Earth. Credit: Blaise Douros/LLNL Lawrence Livermore National Laboratory (LLNL) researchers are developing three-dimensional simulations to examine how a nuclear device could be used to deflect or potentially disrupt an asteroid threatening Earth.